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Santa Cruz Biotechnology tza plasmids

Figure 3. YAP and/or TAZ overexpression promotes the proliferation in LOVO cells. Cell proliferation was analyzed by an MTT assay at 24 h after LOVO cells were transfected with YAP and/or <t>TAZ</t> <t>plasmids.</t> Groups: NC, negative control; YAP mimics, cells transfected with YAP expression plasmids; TAZ mimics, cells transfected with <t>TZA</t> expression plasmids; YAP+TAZ, cells co‑transfected with YAP and TAZ plasmids. Values are expressed as the mean ± standard deviation of three independent experi ments. *P<0.05, **P<0.01. YAP, Yes‑associated protein; TAZ, transcriptional co‑activator with PDZ‑binding motif.

Tza Plasmids, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tza+plasmids/TAZ+CRISPR+Activation+Plasmid/pm29042987-30-24-29
Average 92 stars, based on 1 article reviews

tza plasmids - by Bioz Stars, 2026-09

92/100 stars

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1) Product Images from "Upregulation of Yes-associated protein and transcriptional co-activator with PDZ-binding motif influences the behavior of LOVO human colon adenocarcinoma cells."

Article Title: Upregulation of Yes-associated protein and transcriptional co-activator with PDZ-binding motif influences the behavior of LOVO human colon adenocarcinoma cells.

Journal: Experimental and therapeutic medicine

doi: 10.3892/etm.2017.4962

Figure Legend Snippet: Figure 3. YAP and/or TAZ overexpression promotes the proliferation in LOVO cells. Cell proliferation was analyzed by an MTT assay at 24 h after LOVO cells were transfected with YAP and/or TAZ plasmids. Groups: NC, negative control; YAP mimics, cells transfected with YAP expression plasmids; TAZ mimics, cells transfected with TZA expression plasmids; YAP+TAZ, cells co‑transfected with YAP and TAZ plasmids. Values are expressed as the mean ± standard deviation of three independent experi ments. *P<0.05, **P<0.01. YAP, Yes‑associated protein; TAZ, transcriptional co‑activator with PDZ‑binding motif.

Techniques Used: Over Expression, MTT Assay, Transfection, Negative Control, Expressing, Standard Deviation

Figure 5. YAP and/or TAZ overexpression changes the protein expression of tumor‑ or apoptosis‑associated genes in LOVO cells. At 24 h after the transfection, CTGF, Cyr61, Bcl‑2, Bax and caspase‑3 protein expression levels were determined by using western blot analysis. Groups: NC, negative control; YAP mimics, cells transfected with YAP expression plasmids; TAZ mimics, cells transfected with TZA expression plasmids; YAP+TAZ, cells co‑transfected with YAP and TAZ plasmids. YAP, Yes‑associated protein; TAZ, transcriptional co‑activator with PDZ‑binding motif; CTGF, connec tive tissue growth factor; Cyr61, cysteine‑rich angiogenic inducer 61; Bcl‑2, B‑cell lymphoma 2; Bax, Bcl‑2‑associated X protein.

Figure Legend Snippet: Figure 5. YAP and/or TAZ overexpression changes the protein expression of tumor‑ or apoptosis‑associated genes in LOVO cells. At 24 h after the transfection, CTGF, Cyr61, Bcl‑2, Bax and caspase‑3 protein expression levels were determined by using western blot analysis. Groups: NC, negative control; YAP mimics, cells transfected with YAP expression plasmids; TAZ mimics, cells transfected with TZA expression plasmids; YAP+TAZ, cells co‑transfected with YAP and TAZ plasmids. YAP, Yes‑associated protein; TAZ, transcriptional co‑activator with PDZ‑binding motif; CTGF, connec tive tissue growth factor; Cyr61, cysteine‑rich angiogenic inducer 61; Bcl‑2, B‑cell lymphoma 2; Bax, Bcl‑2‑associated X protein.

Techniques Used: Over Expression, Expressing, Transfection, Western Blot, Negative Control

Figure 4. YAP and/or TAZ over‑expression reduces the apoptosis of LOVO cells. After LOVO cells were transfected with YAP and/or TAZ plasmids for 24 h, flow cytometry was performed for cell apoptosis detection. Groups: NC, negative control; YAP mimics, cells transfected with YAP expression plas mids; TAZ mimics, cells transfected with TZA expression plasmids; YAP+TAZ, cells co‑transfected with YAP and TAZ plasmids. Values are expressed as the mean ± standard deviation of three independent experiments. *P<0.05, **P<0.01. YAP, Yes‑associated protein; TAZ, transcriptional co‑activator with PDZ‑binding motif; FITC, fluorescein isothiocyanate; Q, quadrant.

Figure Legend Snippet: Figure 4. YAP and/or TAZ over‑expression reduces the apoptosis of LOVO cells. After LOVO cells were transfected with YAP and/or TAZ plasmids for 24 h, flow cytometry was performed for cell apoptosis detection. Groups: NC, negative control; YAP mimics, cells transfected with YAP expression plas mids; TAZ mimics, cells transfected with TZA expression plasmids; YAP+TAZ, cells co‑transfected with YAP and TAZ plasmids. Values are expressed as the mean ± standard deviation of three independent experiments. *P<0.05, **P<0.01. YAP, Yes‑associated protein; TAZ, transcriptional co‑activator with PDZ‑binding motif; FITC, fluorescein isothiocyanate; Q, quadrant.

Techniques Used: Transfection, Flow Cytometry, Negative Control, Expressing, Standard Deviation

Transfection:

Article Title: Upregulation of Yes-associated protein and transcriptional co-activator with PDZ-binding motif influences the behavior of LOVO human colon adenocarcinoma cells
Article Snippet: .. To investigate the role of YAP and TAZ in LOVO cells, YAP plasmids (product no. sc-400040-ACT; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and/or TZA plasmids (product no. sc-400320-ACT; Santa Cruz Biotechnology, Inc.) were transfected into LOVO cells with 30 μl Lipofectamine 2000 transfection reagent (Invitrogen; Thermo Fisher Scientific, Inc.) following the manufacturer's instructions. ..

Article Title: Upregulation of Yes-associated protein and transcriptional co-activator with PDZ-binding motif influences the behavior of LOVO human colon adenocarcinoma cells.
Article Snippet: .. To investigate the role of YAP and TAZ in LOVO cells, YAP plasmids (product no. sc-400040-ACT; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and/or TZA plasmids (product no. sc-400320-ACT; Santa Cruz Biotechnology, Inc.) were transfected into LOVO cells with 30 μl Lipofectamine 2000 transfection reagent (Invitrogen; Thermo Fisher Scientific, Inc.) following the manufacturer's instructions. ..

Article Title: Upregulation of Yes-associated protein and transcriptional co-activator with PDZ-binding motif influences the behavior of LOVO human colon adenocarcinoma cells
Article Snippet: .. Cell transfection To investigate the role of YAP and TAZ in LOVO cells, YAP plasmids (product no. sc-400040-ACT; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and/or TZA plasmids (product no. sc-400320-ACT; Santa Cruz Biotechnology, Inc.) were transfected into LOVO cells with 30 μl Lipofectamine 2000 transfection reagent (Invitrogen; Thermo Fisher Scientific, Inc.) following the manufacturer's instructions. ..

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Journal: Experimental and therapeutic medicine

Article Title: Upregulation of Yes-associated protein and transcriptional co-activator with PDZ-binding motif influences the behavior of LOVO human colon adenocarcinoma cells.

doi: 10.3892/etm.2017.4962

Figure Lengend Snippet: Figure 3. YAP and/or TAZ overexpression promotes the proliferation in LOVO cells. Cell proliferation was analyzed by an MTT assay at 24 h after LOVO cells were transfected with YAP and/or TAZ plasmids. Groups: NC, negative control; YAP mimics, cells transfected with YAP expression plasmids; TAZ mimics, cells transfected with TZA expression plasmids; YAP+TAZ, cells co‑transfected with YAP and TAZ plasmids. Values are expressed as the mean ± standard deviation of three independent experi ments. *P<0.05, **P<0.01. YAP, Yes‑associated protein; TAZ, transcriptional co‑activator with PDZ‑binding motif.

Article Snippet: To investigate the role of YAP and TAZ in LOVO cells, YAP plasmids (product no. sc-400040-ACT; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and/or TZA plasmids (product no. sc-400320-ACT; Santa Cruz Biotechnology, Inc.) were transfected into LOVO cells with 30 μl Lipofectamine 2000 transfection reagent (Invitrogen; Thermo Fisher Scientific, Inc.) following the manufacturer's instructions.

Techniques: Over Expression, MTT Assay, Transfection, Negative Control, Expressing, Standard Deviation

Journal: Experimental and therapeutic medicine

Article Title: Upregulation of Yes-associated protein and transcriptional co-activator with PDZ-binding motif influences the behavior of LOVO human colon adenocarcinoma cells.

doi: 10.3892/etm.2017.4962

Figure Lengend Snippet: Figure 5. YAP and/or TAZ overexpression changes the protein expression of tumor‑ or apoptosis‑associated genes in LOVO cells. At 24 h after the transfection, CTGF, Cyr61, Bcl‑2, Bax and caspase‑3 protein expression levels were determined by using western blot analysis. Groups: NC, negative control; YAP mimics, cells transfected with YAP expression plasmids; TAZ mimics, cells transfected with TZA expression plasmids; YAP+TAZ, cells co‑transfected with YAP and TAZ plasmids. YAP, Yes‑associated protein; TAZ, transcriptional co‑activator with PDZ‑binding motif; CTGF, connec tive tissue growth factor; Cyr61, cysteine‑rich angiogenic inducer 61; Bcl‑2, B‑cell lymphoma 2; Bax, Bcl‑2‑associated X protein.

Techniques: Over Expression, Expressing, Transfection, Western Blot, Negative Control

Journal: Experimental and therapeutic medicine

Article Title: Upregulation of Yes-associated protein and transcriptional co-activator with PDZ-binding motif influences the behavior of LOVO human colon adenocarcinoma cells.

doi: 10.3892/etm.2017.4962

Figure Lengend Snippet: Figure 4. YAP and/or TAZ over‑expression reduces the apoptosis of LOVO cells. After LOVO cells were transfected with YAP and/or TAZ plasmids for 24 h, flow cytometry was performed for cell apoptosis detection. Groups: NC, negative control; YAP mimics, cells transfected with YAP expression plas mids; TAZ mimics, cells transfected with TZA expression plasmids; YAP+TAZ, cells co‑transfected with YAP and TAZ plasmids. Values are expressed as the mean ± standard deviation of three independent experiments. *P<0.05, **P<0.01. YAP, Yes‑associated protein; TAZ, transcriptional co‑activator with PDZ‑binding motif; FITC, fluorescein isothiocyanate; Q, quadrant.

Techniques: Transfection, Flow Cytometry, Negative Control, Expressing, Standard Deviation

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